Figure 2.

Targeting a pTHI11::eGFP reporter system with the dCas9 system expressing scgRNAs that target T1–T5 under the control of the methanol inducible pALD4 promoter. Log₂ fold changes (fc) of eGFP fluorescence compared to the NTC. (A) Strains containing the activation domain VP64 fused to MS2 in the absence of thiamine, (B) the same strains as in A cultivated in the presence of 100 μM thiamine, (C) strains without the activation domain (NAC) in the absence of thiamine, and (D) the same strains as in C cultivated in the presence of 100 μM thiamine. Error bars indicate the sum of squared errors of the respective target sequence compared to the NTC. The number of clones is indicated above each bar chart.