Figure 3.

Targeting a pTHI11::eGFP reporter system with the scgRNA/dCas9 system expressing the scgRNA under the control of the strong constitutive pGAP promoter. Log₂ fold changes of eGFP fluorescence compared to the NTC. (A) Strains containing the activation domain VP64 fused to MS2 in the absence of thiamine, (B) the same strains as in A cultivated in the presence of 100 μM thiamine, (C) strains without the activation domain (NAC) in the absence of thiamine. (D–F) the same strains as in (A–C) but cultivated under glucose surplus conditions. Error bars indicate the sum of squared errors of the respective target sequence and the related negative control. Number of clones is indicated on top.