FIGURE 1.

Aloe emodin (AE) protected the integrity of the endothelium and restored blood pressure in vivo. Blood pressure was measured by tail sleeve in mice. (A) and (B) Summarized data showing the systolic blood pressure and diastolic blood pressure (n = 8). (C) and (D): Zonula occludens‐1 (ZO‐1)/PECAM and ZO‐2/PECAM were identified by confocal microscopy with frozen sections of mouse hearts, the merged images displayed yellow dots or patches indicating the colocalization of ZO‐1 or ZO‐2 (green) with PECAM (red). (E) Summarized data showing the colocalization coefficient of ZO‐1 with PECAM (n = 6). (F) Summarized data showing the colocalization coefficient of ZO‐2 with PECAM (n = 6). ^*** P < 0.001, angiotensin II (Ang II) vs. Control, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, AE/Losarten vs. Ang II. Scale bar, 20 µM