FIGURE 2.

Aloe emodin (AE) suppressed angiotensin II (Ang II)‐induced activation of NOD‐like receptor family pyrin domain containing‐3 (NLRP3) inflammasome and high mobility group box1 (HMGB‐1) expression in vivo. (A) Frozen sections of mouse hearts were stained for PECAM, and Alexa 488‐conjugated antibodies against NLRP3 in coronary arteries. The merged images displayed yellow dots or patches indicating the colocalization of PECAM (red) with NLRP3 (green). (B) Summarized data showing the colocalization coefficient of PECAM with NLRP3 (n = 8). (C) and (D): The content of IL‐1β (n = 6) and HMGB1 (n = 7) in serum were detected by ELISA kit. ^*** P < 0.001, Ang II vs. Control, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001, and AE vs. Ang II. Scale bar, 20 µm