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. 2020 Jul 15;97(11):1180–1186. doi: 10.1002/cyto.a.24187

Figure 2.

Figure 2

HEADER and TEXT segments were checked against the FCS specification. A. Offsets were always right aligned either with leading spaces or leading zeros. Absence of ANALYSIS segments was indicated by empty offsets, negative offsets (incorrect) or both offsets being zero. B. Various invalid offset pairs were found, including invalid ranges (start > end), ranges out‐of‐range (end > size) and overlapping ranges (segments having bytes in common). C. Inaccurate TEXT offsets always resulted in additional bytes being included. D. These were mostly padding bytes and/or bytes from the adjacent segment. E. Various delimiters were used. F. Some TEXT segments featured double closing delimiters. G. Others featured no closing delimiter at all. H. Many empty values were identified, despite being strictly prohibited by the FCS specification. I. Escapes were less frequently found, as compared to empty values. J. FlowCore and FlowJo alternately misinterpreted the consecutive delimiters (empty values vs. escapes).