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. 2020 Dec 21;153(2):e202012704. doi: 10.1085/jgp.202012704

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© 2020 Liang and Yang

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Figure 6. — Ca²⁺ binding sites mediate pH_i regulation on TMEM16F. (A) Representative TMEM16F-E667Q currents recorded from inside-out patches perfused with intracellular solutions containing 0.1, 1, 5, 10, and 20 mM Ca²⁺ at different pH_i values. (B) Ca²⁺ dose–response of TMEM16F-E667Q mutation. The error bars represent SEM (n = 4). (C) EC₅₀ values of Ca²⁺ at pH_i 6.1, 7.3, and 8.9 were 8.64 ± 1.08, 2.93 ± 0.42, and 1.22 ± 0.31 mM, respectively. The error bars represent SEM (n = 4). P values were determined with Tukey test comparisons after one-way ANOVA: ***, P < 0.001; *, P < 0.5. (D) The G-pH_i relationship of TMEM16F-E667Q. Solid lines represent linear fits. (E) The pH_i sensitivity and [Ca²⁺]_i concentration relationship of E667Q (solid line). The peak pH sensitivity is 0.22 at ∼3.01 mM Ca²⁺. The error bars represent SEM (n = 4). Curve from WT (dashed line) was replotted here for reference.