Fig. 2.

A. Images of the peroneal nerve in Thy1-YFP mice to show one of the 3 branches of the peroneal nerve (indicated by * in the figure) transected immediately prior to entry into the Tibialis Anterior (TA) muscle leaving 2 branches intact. This image was taken at 7 days post-transection showing lack of any regrowth of the nerve (a). Intact muscles stained with α-bungarotoxin to visualise the AChR showed variability in NMJ structure (b). At 7 days post-surgery, four regions were identified where: all fibers had lost axonal input but retained AChR (R1), all fibers retained full innervation (R3), or fibers had a mix of innervated and some denervated fibers (R2, R4). Reproduced from Ref. [79].

B.The regions identified in Fig. 2A in longitudinal section were identified on transverse sections of the TA muscle and small bundles of fibers were obtained from each region (b). These fibers were permeablised and state 1 mitochondrial peroxide generation examined at 7 (b) days post-surgery in comparison with sham-operated control muscles; *P < 0.05 compared with fibers from the same region of sham-operated muscles. Reproduced from Ref. [79].