Fig. 3.

A.Representative fluorescent images of NMJ's from adult (6–8 months) (A) and old (26 months) (B) Thy1-CFP mice showing the pre-synaptic terminal motor nerves (blue) and motor endplates stained with α-bungarotoxin (red) (scale bar = 50 μm). No significant difference was observed between the cytosolic H₂O₂ content (indicated by the cyto-HyPer2 fluorescence) of transfected AT muscles from adult and old mice when assessing the ratio of emissions at 516 nm after 488 and 405 excitation (488/405 ratio) from individual fibers (C). Mitochondrial peroxide generation monitored by the rate of amplex red oxidation (expressed as H₂O₂ generation) during state 1 respiration (i.e. in the absence of added substrates) from permeablised AT fibers from adult and old mice (D). ***p < 0.001 Reproduced from Ref. [94]. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)