Fig. 6.

A. Proportion of Prx1, 2 and 3 in the oxidised (dimerised) form on denaturing gels from isolated fibers from the FDB of adult and old WT mice subjected to an electrical stimulation protocol for up to 15 min. Fibers were processed at the stated time points by incubation in 75 mM MMTS for 10 min and then lysed in the presence of MMTS. Lysates were subjected to Western blot analysis using non-reducing gels and the Prx antibodies. Results are presented as the percentage of the total Prx signal found in the dimerised form. Graphs show mean ± SEM. *P < 0.05, †P < 0.05 compared to adult baseline values, ƚ P < 0.05 compared to old baseline values.

B. Published data from our group [63] showing the increase in Trx and TrxR in muscle from old compared with adult mice, *P < 0.05, **P < 0.001 compared with adult.