Antibodies |
|
Rabbit polyclonal anti-GABA |
Sigma-Aldrich |
Cat# A2052; RRID: AB_477652
|
Goat anti rabbit IgG, Biotin SP conjugated |
Merck-Millipore |
Cat# AP132B; RRID: 11212148 |
Streptavidin conjugated Alexa Fluor 647 |
Thermo Scientific |
Cat# S21374; RRID: 2336066 |
|
Bacterial and Virus Strains |
|
AAV-DJ-CaMKIIa-C1V1(E162T)-TS-p2A-mCherry-WPRE |
Stanford Vector Core |
GVVC-AAV-46 |
AAV1-Syn-GCaMP6f-WPRE-SV40 |
Addgene, Chen et al., 2013
|
100837-AAV9 |
|
Chemicals, Peptides, and Recombinant Proteins |
|
16% Formaldehyde (w/v), Methanol free (cat:28908) |
Thermo Scientific |
Cat# 28908 |
10X PBS |
Thermo Scientific |
Cat# AM9624 |
Tween 20 |
Sigma-Aldrich |
Cat# P1379 |
Goat Serum |
Merck-Millipore |
Cat# S26-100ML |
|
Experimental Models: Organisms/Strains |
|
Mouse: C57BL/6 |
Charles River Laboratories |
Cat # 632 |
|
Software and Algorithms |
|
ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
MATLAB |
Mathworks |
https://uk.mathworks.com/ |
Suite2p |
Pachitariu et al., 2017 |
https://github.com/MouseLand/suite2p |
Blender |
The Blender Foundation |
https://www.blender.org/ |
Virtual reality code in Python |
https://github.com/neurodroid/gnoom |
N/A |