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The first oxidation-state selective probes of ferrous iron rely on reactivity-based sensing. Three important classes of reactivity-based probes are shown, along with schematic representations of their modes of activation. RhoNox-based probes undergo iron(II)-promoted reduction of an N-oxide to produce a fluorescent signal. TRX-based probes include FRET-based turn-on and ratiometric probes, and caged reporter probes.
