(A) Schematic of polymers P1-P3 and their estimated average lengths[31]; AmPAS12SH – P1, AmPAS25SH – P2, AmPAS50SH – P3. Magnified: structure of repeating polymer subunit. (B) Schematic of SMFS experiment. Magnified: structure of sialic acid. (C) Representative retraction curves obtained from SMFS. D. Average number of adhesion events per curve comparing 2-mercaptoethanol (2ME), GlcPAS, DP 50, and P1-P3 tips over: unmodified mica (negative control), amine silanized (negative control), and mucin functionalized. Data obtained by randomly selecting 5 sets of 20 curves to tabulate average number of events. This was repeated three times to generate an average and standard deviation (error bars) (n.s., p> 0.05; *, p < 0.05; **, p < 0.01, ***, p < 0.001).