Fig. 1.

Dapk2 deficiency in T cells leads to increased GC responses. (A) Expression of Dapk2 mRNA increases upon TCR activation. The mRNA level of Dapk2 is analyzed by qPCR and normalized to the level of Hprt, before and after in vitro activation of T cells through crosslinking of CD3 and CD28. Data are mean ± s.e.m. and representative of two independent experiments. (B) Western blot analysis of Dapk2 protein levels in naïve and in vitro activated CD4⁺ T cells. Data are representative of two independent experiments. (C—E) Adoptive transfer of dsRed OT-2 T cells from WT or Dapk2 knockout mice together MD4 B cells into Tcrb^−/−Tcrd^−/− recipients. Frequencies of transferred GC B, OT-2 T and Tfh cells in the recipient mice are shown as flow cytometry contour plots (Left) and statistical quantifications (Right). Each symbol represents the mean of one experiment, with at least 3 mice in each group per experiment. Symbols connected are from the same experiment. NS, not significant. *P < 0.05 (paired t-test).