Fig. 2.

Dapk2 suppresses cognate T-B cell interactions. OT-2 T cells were stained with TAMRA or PE anti-CD4, and incubated with CMF2HC or ef450 anti-CD19 stained LPS-activated B cells pulsed with different concentration of OVA₃₂₃-peptide. (A) Flow cytometry contour plots (left) and dot plots (Right) showing frequencies of WT or KO OT-2 T cells conjugated to B cells. Contour plots are gated on total CD4⁺ T cells. Data are mean ± s.e.m. from one of four independent experiments. **P < 0.01. (B) Flow cytometry contour plots (left) and dot plots (Right) showing conjugation frequencies of control or Dapk2 overexpressing OT-2 T cells to B cells. Contour plots are gated on total infected (GFP⁺) T cells. Data are mean ± s.e.m. and pooled from six independent experiments. **P < 0.01, ***P < 0.001.