Fig. 3.

Proximal TCR signaling and integrin and SLAM family protein expression in Dapk2 KO T cells are intact. (A) PLCγ (Tyr783) and Erk1/2 (Thr202/Tyr204) phosphorylation in control or Dapk2 shRNA transduced CD4⁺ T cells upon CD3 cross-linking. Data are representative of two independent experiments. (B-D) Calcium flux induced by CD3 crosslinking, expression of LFA-1 (αLβ2, CD11a/CD18), VLA-4 (α4β1, CD49d/CD29) (C), and Slam and Ly108 (D) are comparable in activated WT or KO CD4⁺ T cells. Data are representative of four (B) and two (C, D) independent experiments.