Figure 2.

Rs2070600‐allele influences expression of region of advanced glycosylation end‐product specific receptor (RAGE), and the level of RAGE in failing heart. (A) Comparison of RAGE transcript levels in peripheral blood lymphocytes between rs2070600‐AA allele (n = 10), ‐AG (n = 93) and ‐GG allele carriers (n = 129). (B) Protein extracts of human cardiac tissue from patients with severe HF (n = 5) and control (non‐HF) hearts (n = 3) were normalized to GAPDH levels (GAPDH: glyceraldehyde 3‐phosphate dehydrogenase). Genotypes of the samples were described. (C–D) After transient transfection of pc3.1‐RAGE‐rs2070600‐GG and ‐AA, western blot showed the protein expression of ‐AA was significantly lower than ‐GG both in AC16 and 293 T cells. The data are presented as mean ± SD from three independent experiments.