Figure 4:

Binding of N-Ret-PE to ABCA4 variants. [³H]-retinal was added to ABCA4 variants that were immobilized on an affinity matrix in buffer containing DOPE in the absence (− ATP) or presence of 1 mM ATP (+ ATP). The samples were eluted with ethanol and the [³H] retinoid was measured in a liquid scintillation counter. The data are normalized to WT ABCA4 in the absence of ATP. Data are presented as the mean +/− SD for n≥3 independent experiments.