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. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Biometals. 2019 Sep 20;32(6):829–843. doi: 10.1007/s10534-019-00216-1

Fig. 3.

Fig. 3

ZIP14 protein levels decrease in response to 4 and 16 hour Mn exposure. HepaRG cells were treated with metal for 4 or 16 hours in standard culture conditions. a. Shown are representative Western blots for ZIP14 after 4 (top panel) and 16 hour (bottom panel) treatment with MnCl2. Equal volumes of lysed samples were loaded per lane. ZIP14 band intensities were measured and normalized to GAPDH levels. Graphed are the normalized data at 5, 50, and 100μM Mn where the control is set to 1 (dashed line). Values are mean ± SEM, n=4 biological samples. ZIP14 protein levels in response to Mn exposure show a dose-depended regression at P < 0.05. b. Shown are representative Western blots for FPN, ZNT10, and ZIP8 after 16 hour treatment with MnCl2. Cell lysates were prepared for immunoblotting and equal volumes of lysed samples were loaded per lane. FPN, ZNT10, and ZIP8 band values were each normalized to GAPDH levels. Graphed are the normalized data at 5, 50, and 100μM Mn. The control is set to 1 (dashed line). Values are mean ± SEM, n=4 biological samples. ZIP8, ZnT10, and FPN do not show a dose-dependent regression.