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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: SLAS Discov. 2020 Apr 30;25(8):939–949. doi: 10.1177/2472555220915821

Figure 1. Generation of a mouse model of inducible lamin B1 expression.

Figure 1.

A. Mating strategy to generate TRE-FLAG-LMNB1;Rosa rtTA embryonic fibroblasts. MEFs were isolated from embryos at E13.5, genotyped, and lamin B1 expression verified by Western blot. B. Western blot of MEFs treated with doxycycline (DOX) to overexpress exogenous FLAG-LMNB1. The presence of FLAG causes exogenous lamin B1 to migrate slightly slower than endogenous lamin B1, resulting in a double-band. C. Quantification of Western blots in B. D. Immunofluorescence staining of MEF nuclei with an anti-lamin B1 antibody reveals abnormal folds and wrinkles in the nuclear envelope owing to overexpression of lamin B1. E. Nuclear abnormalities were reversible upon DOX removal. Y-axis shows percentage of abnormal nuclei as quantified by visual examination and manual classification as described in Materials and Methods. Error bars – 95% confidence intervals, *-p<0.05.