Figure 1. Correlating coronavirus peptide reactivity and neutralizing titer of COVID-19 convalescent plasma.

a. 126 COVID-19 convalescent plasma donations underwent functional analysis and antibody profiling via VirScan with a comprehensive coronavirus peptide library. Functionalities included neutralizing titer (NT), antibody dependent cellular cytotoxicity (ADCC), antibody dependent cellular phagocytosis (ADCP), and antibody dependent complement deposition (ADCD). Plasma from 87 pre-pandemic controls were additionally analyzed via VirScan.

b. Convalescent plasma was divided into groups based on NT AUC (Low NT: <40 − n=55, Medium NT: 40 to 160 − n=39, and High NT: ≥160 − n=32). The percentages of samples in each group with reactivity to a particular peptide were plotted according to the peptide’s position along each viral genome. Regions with most consistent reactivity were shaded and were located in S and N. Amino acid residue number is included for S. Full genome plots are shown in Figure S1.

c. Immunodominant regions of CoV2 S are mapped onto the CoV2 S structure. (31) Trimeric spike is colored by S1 subunit and S2 subunit with the entire receptor binding domain (RBD) indicated.

d. Aggregate virus score was calculated as the sum of all log-transformed fold changes of peptides designed for a given virus. Bars with an asterisk indicate convalescent plasma groups that had significantly different scores (two sided Wilcox test, p<0.05)

e. Aggregate virus scores from peptides defining dominant regions.