Skip to main content
. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Curr Pharm Des. 2020;26(29):3530–3545. doi: 10.2174/1381612826666200523175409

Fig. (5). Wheat germ agglutinin (WGA)-gold labeling TEM of dorsal elytral cuticle of T. castaneum.

Fig. (5).

Ultra-thin sections (~90 nm) of pharate adults (5 d-old pupae) that were injected with dsTcVer (200 ng per insect) in late instar larvae were incubated with gold-labeled (10 nm) WGA to detect chitin in the dorsal side of elytral cuticle. dsRNA for chitin synthase-A (dsTcChs-A), which is required for cuticular chitin synthesis, was injected as a negative control. Chitin was detected in both horizontal laminae and vertical PCFs of elytral cuticle from dsTcVer-treated insects (A and C). Few or no gold particles were observed in TcChs-A-deficient insects (B and D). EN, envelope; EP, epicuticle; PRO, procuticle; PCF, pore canal fibers; APMP, apical plasma membrane protrusion. Scale bar in A and B = 1 μm and C and D = 500 nm. From Noh et al., (2015) [22].