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Parent/b8-product ion transitions used for %2Cys quantitation of PRM experiments. The three b8-product ions listed represent the second, third, and fourth isotopes
| Parent ion |
b8-product ion |
||||||
|---|---|---|---|---|---|---|---|
| Lys-C IgG2-A hinge dipeptide | Monoisotopic mass | m/z | z | Isolation width | m/z | z | Mass tolerance |
| Classically linked | 5350.5911 Da | 1071.9241 | +5 | 3 Da | 1169.8431, 1170.1743, 1170.5077 | +3 | 10 ppm |
| Doubly cysteinylated | 5590.6160 Da | 1119.9241 | +5 | 3 Da | 1249.8461, 1250.1804, 1250.5151 | +3 | 10 ppm |
