Skip to main content
. 2020 Dec 22;15(12):e0243812. doi: 10.1371/journal.pone.0243812

Fig 3. Effects of TFR1 on the malignant behaviours of CSCs.

Fig 3

A. Fluorescence was observed 48 h after shRNA transfection. Images were obtained using a 100× objective. B. TFR1 knockdown efficacy was measured by performing western blotting. *P<0.05, vs. shScrambled-transfected cells. After TRF1 knockdown in CSCs derived from Huh-7 and SK-HEP-1 cells, malignant behaviours, including cell cycle distribution (C), invasive ability (D) and tumour formation in soft agar (E), were measured. *P<0.05, vs. shScrambled-transfected cells. Images were obtained using a 100× objective.