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. 2020 Dec 22;15(12):e0243812. doi: 10.1371/journal.pone.0243812

Fig 4. TFR1 accumulates intracellular iron in CSCs.

Fig 4

A. Intracellular iron levels were measured 48 h later TFR1 knockdown. *P<0.05, vs. shScrambled-transfected cells. B. After the addition of 10 μg/ml of FAC, the intracellular iron levels were measured. *P<0.05, vs. shScrambled-transfected cells. #P<0.05, vs. shScrambled-transfected+FAC cells. C. The level of chelatable intracellular iron was measured with RPA. Images were obtained using a 100× objective. *P<0.05, vs. shScrambled-transfected cells.