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A. After TFR1 knockdown, sphere formation under the presence of 0.2 or 0.8 μM erastin was observed. Images were obtained using a 40× objective. B. After TFR1 knockdown, cell viability was measured in the presence of 0.2 or 0.8 μM erastin by performing the CCK-8 assay. *P<0.05, vs. the Mock group; **P<0.01, vs. the Mock group. C. Cell viability was measured in the presence of DFO, a ferroptosis inhibitor. *P<0.05, vs. the shScrambled group.
