Fig 3. Increased PI3K/AKT signalling in Cul7-depleted cardiac myocytes.

Lysates of CM isolated from Cul7^flox/floxAAV9-CMV-iCre mice at 8 weeks of age were subjected to SDS-PAGE and Western blot analysis using antibodies directed against AKT ^pS473 (A), IRS-1 (B), ERK ^pT202/pY204 (C) and HSP90. Data are shown as fold change normalized to CM of age- and sex-matched Cul7^flox/floxAAV9-CMV-dsRed mice and expressed as mean ± SEM. n = 4-7/group. **p<0.01 (unpaired t-test).