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Primers used for the pMOO-32 multiplex PCR
| Primer | Sequence (5′–3′) | Product size (bp) | Target |
|---|---|---|---|
| aph(3′)-lla_F | TGGCTACCCGTGATATTGCT | 642 | aph(3′)-lla/aph(6)-Ic junction |
| aph(6)-lc_R | CTGGCGGACGGGAAGTATC | ||
| HI2A_F | AGCCTTTCTCACGGTAGCAT | 526 | HI2 repA |
| HI2A_R | TTCAATTGTCGGTGAGCGTC | ||
| TraI_F | CGGGAAAAGCTGCACTCAAT | 396 | traI |
| TraI_R | AAGACTTTGTGAGCTTGGCG | ||
| TetB_F | TTCAGCGCAATTGATAGGCC | 285 | tetB |
| TetB_R | ATCCCACCACCAGCCAATAA | ||
| CTX-M-32_F | TTAGGAAGTGTGCCGCTGTA | 180 | blaCTX-M-32 |
| CTX-M-32_R | CACGGCCATCACTTTACTGG |
