TABLE 2.

Selected bacterial families in cecal digesta of pigs fed diets with or without phytase and lactic acid treatment of cereals^a

Family	Mean % relative abundance under indicated grain treatment conditions				SEM	P value
	No phytase		Phytase
	Con	LA	Con	LA		Phytase	LA	Phytase × LA
Prevotellaceae	32.40	35.23	27.42	28.19	2.035	0.007	0.385	0.617
Bacteroidaceae	13.41	11.32	12.74	12.10	0.419	0.898	0.003	0.096
Clostridiaceae	8.16 bc	6.49 c	8.60 ab	10.20 a	0.729	0.009	0.960	0.035
Ruminococcaceae	5.80	5.21	6.73	6.84	0.457	0.010	0.609	0.457
Veillonellaceae	3.93 b	5.97 a	3.64 b	4.02 b	0.322	0.002	0.001	0.016
Lachnospiraceae	3.96	3.49	4.83	4.58	0.395	0.020	0.373	0.778
Porphyromonadaceae	2.15	1.61	2.14	1.99	0.105	0.088	0.003	0.074
Bacillaceae	1.06	1.03	1.19	1.28	0.057	0.003	0.668	0.277
Erysipelotrichaceae	0.88 b	0.86 b	1.00 b	1.32 a	0.076	0.001	0.055	0.037
Acidaminococcaceae	0.77	0.92	0.71	0.71	0.044	0.005	0.086	0.119
Streptococcaceae	0.76	0.51	0.87	0.60	0.108	0.381	0.026	0.935
Unclassified Clostridiales	0.67	0.52	0.80	0.75	0.073	0.020	0.167	0.494
Flavobacteriaceae	0.74	0.60	0.72	0.66	0.026	0.388	0.001	0.112
Thermoanaerobacteraceae	0.53	0.53	0.60	0.61	0.026	0.014	0.798	0.807
Campylobacteraceae	0.68	0.84	0.56	0.24	0.162	0.037	0.623	0.155
Fusobacteriaceae	0.441	0.403	0.476	0.491	0.022	0.010	0.600	0.237
Enterobacteriaceae	0.467	0.473	0.333	0.313	0.037	0.001	0.863	0.728
Thermoanaerobacterales III	0.350	0.327	0.397	0.404	0.020	0.005	0.710	0.473
Cytophagaceae	0.311	0.258	0.303	0.276	0.011	0.627	0.001	0.248
Paenibacillaceae	0.252	0.236	0.283	0.292	0.016	0.013	0.828	0.434
Pasteurellaceae	0.292	0.234	0.212	0.206	0.025	0.042	0.217	0.302
Clostridiales XI	0.224	0.196	0.250	0.255	0.012	0.002	0.360	0.197
Enterococcaceae	0.203	0.192	0.229	0.242	0.012	0.003	0.948	0.293
Fibrobacteraceae	0.213	0.173	0.208	0.195	0.011	0.435	0.025	0.254
Chlorobiaceae	0.193	0.172	0.194	0.186	0.004	0.124	0.004	0.187
Helicobacteraceae	0.210	0.238	0.170	0.110	0.031	0.011	0.607	0.165
Vibrionaceae	0.217	0.172	0.156	0.146	0.017	0.020	0.124	0.332
Sphingobacteriaceae	0.182	0.142	0.182	0.163	0.008	0.192	0.001	0.194
Thermotogaceae	0.151	0.135	0.162	0.166	0.008	0.016	0.509	0.226
Syntrophomonadaceae	0.145	0.141	0.165	0.160	0.008	0.021	0.591	0.961
Burkholderiaceae	0.177	0.129	0.139	0.131	0.013	0.172	0.035	0.134
Aerococcaceae	0.136	0.113	0.159	0.153	0.013	0.022	0.282	0.528
Staphylococcaceae	0.119	0.117	0.138	0.150	0.007	0.001	0.497	0.328
Rikenellaceae	0.144	0.101	0.142	0.120	0.008	0.317	0.001	0.228
Shewanellaceae	0.148	0.123	0.116	0.110	0.009	0.020	0.096	0.305
Pseudomonadaceae	0.144	0.119	0.119	0.113	0.006	0.019	0.022	0.164
Listeriaceae	0.110	0.109	0.124	0.132	0.005	0.001	0.524	0.403
Neisseriaceae	0.124 a	0.076 b	0.084 b	0.073 b	0.008	0.014	0.001	0.032
Rhodobacteraceae	0.092	0.077	0.089	0.084	0.004	0.706	0.027	0.228
Halanaerobiaceae	0.080	0.081	0.089	0.090	0.004	0.031	0.800	0.987
Comamonadaceae	0.098	0.073	0.081	0.076	0.006	0.242	0.021	0.113
Unclassified Sphingobacteriales	0.087	0.073	0.084	0.078	0.003	0.704	0.004	0.189
Aeromonadaceae	0.104	0.083	0.054	0.048	0.014	0.005	0.348	0.591
Mycoplasmataceae	0.064	0.058	0.071	0.079	0.003	0.000	0.865	0.067
Unclassified Bacillales	0.062	0.059	0.071	0.075	0.004	0.002	0.781	0.330
Bradyrhizobiaceae	0.069	0.061	0.068	0.064	0.003	0.860	0.041	0.502
Peptostreptococcaceae	0.062 ab	0.051 b	0.065 a	0.074 a	0.005	0.013	0.765	0.049
Moraxellaceae	0.074	0.058	0.059	0.057	0.004	0.034	0.026	0.079
Unclassified Bacteroidetes	0.069	0.046	0.070	0.062	0.005	0.082	0.002	0.114
Alteromonadaceae	0.068	0.053	0.052	0.051	0.004	0.022	0.032	0.054
Rhodospirillaceae	0.064	0.052	0.056	0.047	0.005	0.181	0.031	0.763
Unclassified Burkholderiales	0.062	0.043	0.055	0.048	0.004	0.871	0.006	0.145

^aValues are presented as least-square means ± SEM (n = 8 pigs per dietary treatment). Different lowercase letters (a, b, and c) in cell entries within a row indicate a significant difference (P ≤ 0.05). Con, control; LA, lactic acid-treated cereals. Only families that showed relative abundances of >0.05% of all reads and that were significantly different or tended to be different among treatments are presented. Sequence data were analyzed before April 2020, when the family Lactobacillaceae was reclassified (52). Due to this reclassification, Leuconostocaceae were united with Lactobacillaceae, becoming the novel Lactobacillaceae family. Accordingly, data from Lactobacillaceae and Leuconostocaceae were merged.