TABLE 2.
Numbers of aromatic hydrocarbon- and alkane-degrading genes harbored in the major bacterial MAGs detected in the consortium
| Enzyme | No. of genes in genus: |
|||||
|---|---|---|---|---|---|---|
| Pseudomonas | Sphingobium | Ochrobactrum | Achromobacter | Cupriavidus | Parvibaculum | |
| Aromatic ring-hydroxylating dioxygenase | ||||||
| Class A (benzene/naphthalene/PAH-dioxygenases) | 0 | 3 | 0 | 0 | 0 | 1 |
| Class B (benzoate/anthranilate-dioxygenases) | 2 | 1 | 0 | 2 | 1 | 1 |
| Class C (anthranilate dioxygenase/salicylate hydroxylase) | 0 | 3 | 0 | 1 | 2 | 0 |
| Aromatic ring-cleaving dioxygenase | ||||||
| 1,2-Dihydroxynaphthalene dioxygenase (nahC) [EC 1.13.11.56] | 0 | 1 | 0 | 1 | 0 | 1 |
| Catechol 1,2-dioxygenase (catA) [EC 1.13.11.1] | 1 | 1 | 0 | 1 | 1 | 0 |
| Catechol 2,3-dioxygenase (xylE) [EC 1.13.11.2] | 0 | 2 | 1 | 1 | 1 | 0 |
| Protocatechuate 3,4-dioxygenase (pcaGH) [EC 1.13.11.3] | 1 | 0 | 1 | 0 | 1 | 0 |
| Gentisate 1,2-dioxygenase (nagI) [EC 1.13.11.4] | 1 | 1 | 0 | 2 | 2 | 1 |
| Aromatic ring-monooxygenase | ||||||
| Phenol/toluene 2-monooxygenase (dmpKLMNOP) [EC 1.14.13.243] | 0 | 1 | 0 | 0 | 1 | 0 |
| Toluene methyl-monooxygenase (xylM) [EC 1.14.15.26]a | 0 | 1 | 0 | 0 | 0 | 0 |
| Alkane monooxygenase | ||||||
| Alkane 1-monooxygenase (alkB) [EC 1.14.15.3] | 2 | 1 | 0 | 0 | 0 | 0 |
| Putative CYP153 family alkane monooxygenase | 0 | 1 | 0 | 0 | 0 | 5 |
| Putative flavin-binding alkane monooxygenase (almA) | 2 | 0 | 0 | 0 | 1 | 2 |
a
Oxidation of the methyl group on the aromatic ring.