FIG 2.

Biochemical characterization of HcbA1A3. (a) Effect of molar ratios of HcbA1 to HcbA3 on hexachlorobenzene (HCB) consumption. (b) Effect of reaction duration on HCB consumption. The ordinate shows the percentages of residual HCB with respect to the initial HCB level. (c) Effect of temperature on the enzyme activity of HcbA1A3. (d) Effect of pH on the enzyme activity of HcbA1A3. The gray line represents citrate buffer, the blue line represents MES buffer, the red line represents HEPES buffer, the orange line represents Tris-HCl buffer, and the green line represents glycine buffer.