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Substrate-enzyme binding affinity analysis by ForteBio Octet. (a) HCB, PCB, 1,2,3,4-TeCB, and 1,2,4,5-TeCB were used to test their binding affinities with wild-type HcbA1. (b) HCB was used to test its binding affinities with wild-type HcbA1, as well with as its mutants H17A, M12A, R311L, R314L, H79A, V59A, and F10A.
