Table 2. DEG related to the immune infection-defense response at 2 hpe.
| Cx. pipiens SEQ ID | Padj | FC | Sequence length | Blastx results: Best match [Species]* | Conserved domain | E value | IDB.ID accession number | Predicted process/activity | References |
|---|---|---|---|---|---|---|---|---|---|
| Cpip1A029893 | 4.12E-04 | -53.8 | 581 NT | XP_001842114.1 serine protease [Culex quinquefasciatus]** | Lectin_C (pfam00059); CLECT(cd00037) | 1.48E-08; 5.21E-10 | Cpip: CTL24 | Pathogen recognition. C-type lectin (CTL24) | [55–57] |
| Cpip1A025203 | 6.06E-04 | -39.5 | 1877 NT | XP_001846442.1 tar RNA binding protein [Culex quinquefasciatus] | No pfam; DSRM (cd00048) | 3.82E-05 | Cpip: SRRP7 | miRNA pathway; SRRPs: Small Regulatory RNA Pathway Members (LOQS (R3D1)) | [58] |
| Cpip1A088100 | 2.34E-03 | -23.9 | 512 NT | XP_001854407.1 gawky [Culex quinquefasciatus] | Not found | - | RNAi | [59] | |
| Cpip1A057434 | 2.00E-91 | -19.26 | 674 NT | XP_001846424.1 proteasome maturation protein [Culex quinquefasciatus] | UMP1 (pfam05348) | 1.09E-19 | - | Proteasome assembly; | [60,61] |
| Cpip1A048037 | 1.31E-02 | -17.6 | 1416 NT | XP_001842183.1 nuclear pore complex protein Nup155 [Culex quinquefasciatus] | nucleoporin N superfamily (pfam08801) | 2.51E-38 | - | Nuclear transport of transcription factors | [53] |
| Cpip1A079710 | 1.59E-05 | -14.1 | 2229 NT | XP_001866714.1 UBX domain-containing protein 8 [Culex quinquefasciatus] | UBX (pfam00789);UAS_ETEA (cd02991); Faf1_like2_UBX (cd01774); UBA_FAF2 (cd14414) | 0.000121; 7.96E-41; 2.59E-23; 1.08E-10 | - | Ubiquitination pathway | [62] |
| Cpip1A031059 | 8.51E-03 | -12.3 | 3014 NT | XP_001843599.1 ribonuclease 3 [Culex quinquefasciatus] | Ribonuclease_3 (pfam00636); RIBOc (cd00593) | 4.41E-16; 5.19E-20 | Cpip: SRRP6 | miRNA pathway; SRRPs: Small Regulatory RNA Pathway Members (DROSHA) | [63] |
| Cpip1A009744 | 1.4E-02 | -11.6 | 574 NT | XP_001842945.1 defensin-A [Culex quinquefasciatus] | Defensin_2 (pfam01097) | 5 39E-12 | Cpip: AMP6 | Toll/ IMD Pathways. AMP: Antimicrobial Peptide (DEFA) | [64] |
| Cpip1A014968 | 4.31E-02 | -11.2 | 487 NT | AAEL003832-PA [Aedes aegypti] | Defensin_2 (pfam01097) | 1.12E-06 | Cpip: AMP6 | Toll/ IMD Pathways. AMP: Antimicrobial Peptide (DEFC) | [64] |
| Cpip1A005512 | 3.00E-69 | -10.4 | 1499 NT | XP_021702392.1 leucine-rich repeats and immunoglobulin-like domains protein 3 isoform X2 [Aedes aegypti] | LRR_8 (pfam13855); LRR_RI (cd00116) | 2.54E-05; 5.67E-04 | - | Toll/ IMD Pathways/ Pattern Recognition Receptor | [65,66] |
| Cpip1A075565 | 5.55E-06 | -8.3 | 3034 NT | XP_001866729.1 CTP synthase [Culex quinquefasciatus] | CTP_synth_N (pfam06418); CTGs (cd03113) | 4.91E-85; 2.45E-80 | - | Sustainability of activated immune cell proliferation | [54] |
| Cpip1A027127 | 2.00E-169 | -8.1 | 1378 NT | XP_001846690.1 tnf receptor associated factor [Culex quinquefasciatus] | zf-TRAF (pfam02176) | 3.87E-10 | - | RNAi; Apoptosis | [67,68] |
| Cpip1A053625 | 1.00E-175 | -5.0 | 1238 NT | XP_001842520.1 proteasome subunit alpha type 1 [Culex quinquefasciatus] | Proteasome (pfam00227); proteasome_alpha_type_1 (cd03749) | 9.77E-37; 8.16e-109 | - | Proteasome assembly | [69] |
| Cpip1A073928 | 0.00E+00 | -4.6 | 2802 NT | XP_001846422.1 apoptosis inhibitor [Culex quinquefasciatus] | API5 (pfam05918) | 1.89E-130 | - | Apoptosis | [70] |
| Cpip1A002183 | 8.04E-03 | -4.5 | 2885 NT | XP_001866729.1 CTP synthase [Culex quinquefasciatus] | CTP_synth_N (pfam06418); CTGs (cd03113) | 2.54E-85; 1.35E-80 | - | Sustainability of activated immune cell proliferation | [54] |
| Cpip1A091918 | 5.20E-03 | -3.4 | 2066 NT | XP_001862491.1 piwi [Culex quinquefasciatus] | Piwi (pfam02171); Piwi_piwi-like_Euk (cd04658) | 2.83E-58; 1.55E-124 | Cpip:SRRP33 | RNAi; SRRPs: Small Regulatory RNA Pathway Members (PIWI4 transcript 1) | [71,72] |
| Cpip1A087985 | 2.99E-02 | 4.0 | 2946 NT | KXJ72819.1 hypothetical protein RP20_CCG017164 [Aedes albopictus] | PBD (pfam00786); Pkinase (pfam00069); STKc_PAK3 (cd06656) | 4.91E-11; 3.92E-47; 7.99E-116 | - | Not found immunity function reference. PKC-dependent signaling is a negative regulator of the midgut epithelial barrier | [73] |
| Cpip1A058921 | 2.23E-02 | 8.3 | 3952 NT | XP_001843328.1 lipin-3 [Culex quinquefasciatus] | LNS2 (pfam08235); Lipin_N (pfam04571); Lipin_mid (pfam16876) | 3.40E-100; 6.24E-43; 2.32E-20 | - | PI3K-Akt-TOR signaling pathway | [74] |
| Cpip1A000671 | 2.78E-05 | 10.3 | 1398 NT | XP_001844338.1 conserved hypothetical protein [Culex quinquefasciatus] | No pfam; P53 (cd08367) | 2.70E-21 | - | Apoptosis (P53) | [75,76] |
| Cpip1A039204 | 1.99E-02 | 11.1 | 689 NT | XP_001844330.1 conserved hypothetical protein [Culex quinquefasciatus] | E1_DerP2_DerF2 (pfam02221); Npc2_like (cd00916) | 3.13E-13; 8.65E-19 | Cpip: ML12 | Toll/ IMD pathways; MLs: MD2-Like Receptors (ML22) | [77–80] |
| Cpip1A010903 | 3.96E-02 | 15.5 | 1221 NT | XP_001862507.1 ficolin-2 [Culex quinquefasciatus] | Fibrinogen_C (pfam00147); FReD (cd00087) | 8.77E-28; 5.02E-43 | Cpip: FREP46 | Pattern Recognition Receptor | [81] |
| Cpip1A101513 | 8.00E-92 | 18.5 | 661 NT | XP_001846424.1 proteasome maturation protein [Culex quinquefasciatus] | UMP1 (pfam05348) | 8.49E-20 | - | Proteasome assembly; Apoptosis | [60,61] |
| Cpip1A065392 | 1.83E-02 | 25.4 | 1135 NT | XP_001843329.1 conserved hypothetical protein [Culex quinquefasciatus] | Prefoldin (pfam02996); Prefoldin_alpha (cd00584) | 1.21E-06; 6.11E-05 | - | RNAi; Virus infection control | [82] |
| Cpip1A059920 | 2.64E-02 | 25.7 | 1673 NT | XP_001841680.1 chitinase [Culex quinquefasciatus] | Glyco_hydro_18 (pfam00704); CBM_14 (pfam01607); GH18_chitolectin_chitotriosidase (cd02872) | 2.79E-64; 1.13E-10; 7.50E-127 | - | [83] | |
| Cpip1A019311 | 3.37E-02 | 27.5 | 668 NT | XP_001844320.1 MPA2 allergen [Culex quinquefasciatus] | E1_DerP2_DerF2 (pfam02221); Npc2_like (cd00916) | 2.99E-19; 2.94E-26 | Cpip:ML8 | Toll/ IMD pathways; MLs: MD2-Like Receptors (ML6) | [77–80] |
| Cpip1A012593 | 5.05E-03 | 27.6 | 933 NT | XP_001844321.1 conserved hypothetical protein [Culex quinquefasciatus] | E1_DerP2_DerF2 (pfam02221); Npc2_like (cd00916) | 3.72E-11; 5.23E-09 | Cpip:ML16 | Toll/ IMD pathways; MLs: MD2-Like Receptors (ML26) | [77–80] |
| Cpip1A000929 | 4.04E-02 | 28.4 | 3386 NT | XP_001843517.1 fork head [Culex quinquefasciatus] | Forkhead (pfam00250); FH (cd00059) | 1.34E-29; 3.47E-31 | - | TOR (target of rapamycin); Toll and IMD pathways | [84] |
| Cpip1A054937 | 3.90E-02 | 31.3 | 1894 NT | XP_001865865.1 tetraspanin 97e [Culex quinquefasciatus] | Not found | Virus cell-cell spreading. Apoptosis | [85,86] | ||
| Cpip1A037043 | .,68E-02 | 37.3 | 1131 NT | XP_001846625.1 serine protease1/2 [Culex quinquefasciatus] | Trypsin (pfam00089); Tryp_SPc (cd00190) | 6.46E-46; 2.42E-50 | - | Blood digestion | [87] |
| Cpip1A035296 | 1.43E-05 | 37.3 | 1509 NT | XP_001844339.1 conserved hypothetical protein [Culex quinquefasciatus] | No pfam; P53 (cd08367) | 3.22E-21 | - | Apoptosis (P53) | [75,76] |
| Cpip1A014760 | 7.78E-03 | 48.3 | 4632 NT | XP_001843517.1 fork head [Culex quinquefasciatus] | Forkhead (pfam00250); FH (cd00059) | 1.85E-29; 4.78E-31 | - | TOR (target of rapamycin); Toll and IMD pathways | [84] |
| Cpip1A061691 | 4.61E-06 | 74.2 | 939 NT | XP_001846625.1 serine protease1/2 [Culex quinquefasciatus] | Trypsin (pfam00089); Tryp_SPc (cd00190) | 1.66E-46; 2.04E-51 | - | Blood digestion | [87] |
| Cpip1A083737 | 2.16E-03 | 79.7 | 898 NT | XP_001849502.1 chymotrypsin-2 [Culex quinquefasciatus] | Trypsin (pfam00089); Tryp_SPc (cd00190) | 5.28E-51; 7.84E-54 | - | Blood digestion | [87] |
| Cpip1A018924 | 2.19E-03 | 83.1 | 448 NT | XP_001862840.1 croquemort [Culex quinquefasciatus] | CD36 (pfam01130) | 1.05E-28 | Cpip:SCR17 | Apoptosis/Phagocytosis. SCRs: Scavenger Receptors, Class B (SCRBQ3) | [88] |
* First match resulting from the BLASTX search; FC: Fold change; -: no accession number.
**XP_001842114.1 is not a serine protease but a C-type lectin, probable annotation mistake.