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. 2020 Dec 18;13:12965–12977. doi: 10.2147/OTT.S279710

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© 2020 Lin et al.

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Circ_0102537 inhibits the migration and invasion of H1299 cells in vitro. (A) qRT-PCR was performed to verify the expression of circ_0102537 in the human LUAD cell lines. (B) H1299 cells were transfected with si-NC, si-circ_0102537#1, si-circ_0102537#2 or si-circ_0102537#3. qRT-PCR assays confirmed that the transfection was successful. Wound healing assay (C) to assess cell migration. Transwell assay (D) measured invasion of Matrigel by H1299 cells transfected with si-NC or si-circ_0102537#1. H1299 transfected with si-NC or si-circ_0102537#1 were subjected to qRT-PCR (E) and immunofluorescence (F) assays to measure the expression levels of EMT markers. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.