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. 2020 Dec 22;9:e58242. doi: 10.7554/eLife.58242

Figure 4. Treatment of Puumala virus (PUUV) virus-like particles (VLPs) with Fab P-4G2 results in additional density and is associated with loss of continuous lattice at the VLP surface.

Cryo-ET reconstructions of the Fab P-4G2-treated PUUV VLP surface, derived from (A) regions of continuous lattice (14.3 Å) and (B) regions of incomplete lattice (13.4 Å). While both reconstructions show the canonical Gn−Gc architecture (density colored white) and the viral lipid bilayer (light blue), additional density is observed in the latter reconstruction (dark gray). (C) The hantaviral surface carries tetragonal (Gn−Gc)4 spikes that can organize in patches of ordered lattice. (D) A box plot describing the frequency of (Gn−Gc)4 spikes that have a given number of lattice compatible neighbors, from zero to a maximum of eight, shows that treatment with Fab P-4G2 alters the presentation of the (Gn−Gc)4 spike assemblies at the VLP surface.

Figure 4.

Figure 4—figure supplement 1. The Puumala virus (PUUV) virus-like particle (VLP) surface displays ordered regions of glycoprotein lattice and is congruent with previously published reconstructions.

Figure 4—figure supplement 1.

Size-distribution plot of the VLPs (upper right corner) shows that the pleomorphic particles vary in size, with most particles measuring between 80 and 100 nm in the longest dimension. Despite the variable resolutions of the present reconstructions of hantaviral surfaces, the tetragonal lattice organization observed in PUUV VLP (13.9 Å) and in PUUV VLP treated with P-4G2 (14.3 Å) is similar to that of Tula virus (TULV, 15.6 Å, EMDB-4867) (Li et al., 2016) and Hantaan virus (HTNV, 25 Å, EMDB-2056) (Battisti et al., 2011). A top view of each viral surface reconstruction is shown, and densities corresponding to viral membrane and envelope glycoproteins are rendered in light blue and shades of gray and white, respectively.
Figure 4—figure supplement 2. Treatment with Fab P-4G2 alters the presentation of the hantaviral glycoprotein lattice at the surface of Puumala virus (PUUV) virus-like particles (VLPs).

Figure 4—figure supplement 2.

Tomographic slices of the selected PUUV VLPs (left) and corresponding Mercator projections (right) describing the positions of (Gn−Gc)4 spikes on VLP surfaces are shown (A) in the absence and (B) presence of Fab P-4G2. The subset of spike positions that belong to regular patches (determined using the PatchFinder script; please see main Methods) are displayed in black and the remainder in gray. Units shown are in nanometers and scale bars are 50 nm.