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| Antigen | Species | Dilution | Provider | Protocol specifications |
|---|---|---|---|---|
| IGF-1 | Rabbit | 1 : 100 | Abcam (ab263903) | 10 mM sodium citrate, pH = 6 before incubation with blocking solution |
| PAPP-A | Mouse | 1 : 500 | Abcam (ab52030) | 0.1% Triton in PBS, 10 minutes before incubation with blocking solution |
| STC-2 | Rabbit | 1 : 300 | Abcam (ab261915) | — |
