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. 2020 Dec 9;8:598205. doi: 10.3389/fcell.2020.598205

FIGURE 3.

FIGURE 3

Loss of PD-L1 Induces Cell proliferation and Triggers EMT in Aggressive EC Cells. (A) Western blotting analysis of PD-L1 and MCL-1 expression in SPAC-1-L cells overexpressing PD-L1, and in PD-L1-silenced HEC-50 cells. (B) Cellular morphology of HEC-50 cells after knockdown of PD-L1. Scale bar, 100 μm. (C–E) Proliferation (C), wound-healing (D), and invasion (E) assays in EC cells after overexpression or knockdown of PD-L1. (F) A heatmap showing gene expression levels in human aggressive EC cell lines (Expression Atlas database). (G,H) Examination of gene expression in HEC-50 cells after knockdown of PD-L1 (G) and in SPAC-1-L cells after overexpression of PD-L1 (H) was performed using qRT-PCR assays. VIM, Vimentin. ∗P < 0.05.