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. 2020 Dec 9;8:601212. doi: 10.3389/fcell.2020.601212

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Copyright © 2020 Siengdee, Oster, Reyer, Viergutz, Wimmers and Ponsuksili.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tissue collection and isolation of porcine synovial mesenchymal stem cells (SMSCs). (A,B) Synovial tissues were harvested from German Landrace (DL) and Angeln Saddleback (AS) pigs’ stifle joints. (C,D) Fibrous synovium (FS) was harvested from the inner side of the lateral joint capsule; the suprapatellar bursa and adipose synovium (FP) were harvested from the inner side of the infrapatellar fat pad of the knee joint. (E–G) Synovial tissues were washed, chopped, and digested with collagenase. (H–K) After centrifugation, the cell suspension fraction was strained. The cell pellets were then resuspended in the growth medium and cultured.