KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal anti-Fas2 (mouse) | DSHB | 1D4 |
| Monoclonal anti-Fas3 (mouse) | DSHB | 7G10 |
| Monoclonal anti-α-tubulin-FITC (mouse) | Sigma | F2168 |
| Polylonal anti-Insc (rabbit) | Jürgen Knoblich, [53] | N/A |
| Monoclonal anti-Nrg (mouse) | Michael Hortsch, [54] | N/A |
| Polyclonal anti-NrxIV (rabbit) | Christian Klämbt, [55] | N/A |
| anti-Centrosomin (rabbit) | Jordan Raff, [56] | N/A |
| Goat-anti-mouse-488 | ThermoFisher | A-11001 |
| Goat-anti-rabbit-488 | ThermoFisher | A-11008 |
| Goat-anti-mouse-633 | ThermoFisher | A-21050 |
| Goat-anti-rabbit-633 | ThermoFisher | A-21071 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FITC Phalloidin | ThermoFisher | F432 |
| Rhodamine Phalloidin | ThermoFisher | R415 |
| Phalloidin 633 | ThermoFisher | A22284 |
| Vectasheild with DAPI | Vector Labs | H-1200 |
| Experimental Models: Organisms/Strains | ||
| D. Melanogaster: Nrg14 | [57] | FBal0013169 |
| D. Melanogaster: Fas2G0336 | [58] | FBal0152502 |
| D. Melanogaster: β-spectrinFY18 | [30] | FBal0325726 |
| D. Melanogaster: RFP-nls, hsflp, FRT19A | N/A | N/A |
| D. Melanogaster: NrgWT on II | [17] | N/A |
| D. Melanogaster: Nrg167ΔFIGQY on II | [17] | FBal0291693 |
| D. Melanogaster: Nrg180ΔFIGQY on II | [17] | FBal0291694 |
| D. Melanogaster: UAS Inscuteable | [53] | FBtp0006279 |
| D. Melanogaster: UAS myristoylated-RFP | [59] | FBtp0017423 |
| D. Melanogaster: UAS Fas2-YFP | [10] | FBtp0051931 |
| D. Melanogaster: UAS Fas2-Extracellular-YFP | [10] | FBtp0051932 |
| D. Melanogaster: UAS Fas2-Intracellular-YFP | [10] | FBtp0051933 |
| D. Melanogaster: UAS βHeavy-spectrin shRNA (HMS00882) | [60] | FBti0140589 |
| D. Melanogaster: UAS NrxIV shRNA (JF03142) | [60] | FBti0127279 |
| D. Melanogaster: UAS Fas3 shRNA (HMC06528) | [60] | FBti0196009 |
| D. Melanogaster: UAS Ank shRNA (GD10431) | [61] | FBal0198724 |
| D. Melanogaster: Traffic Jam-GAL4 | [62] | FBti0034540 |
| D. Melanogaster: GR1-GAL4 | [63] | FBti0040373 |
| D. Melanogaster: nubbin-GAL4 | [64] | FBti0016825 |
| D. Melanogaster: actin5c-FLPout-GAL4 | N/A | N/A |
| D. Melanogaster: Neuroglian::YFP | [65] | N/A |
| D. Melanogaster: Basigin::YFP | [65] | N/A |
| D. Melanogaster: NrxIV::eGFP | [66] | FBti0099828 |
| Software and Algorithms | ||
| FIJI (1.53) | [50] | https://fiji.sc/ |
| Anaconda3 (Python 3.7) | N/A | https://www.anaconda.com/ |
| GraphPad Prism (8) | N/A | https://www.graphpad.com/scientific-software/prism/ |
| TCoffee | N/A | http://tcoffee.crg.cat/ |