Table 2:

Bunyavirus reverse genetics approaches for live-attenuated vaccines

Approach	Virus	Tested animal model	Refs
Mutation/Deletions	SBV	Cattle	[52]
	RVFV	Mice, sheep, pregnant ewes, marmoset non-human primate (NHP)	[4,48–51]
	SFTSV	Aged-ferret	[44]
Codon-deoptimization	LCMV	Mice	[53,55]
	LASV	Guinea pig	[54]
Single-cycle replicable virus	LASV	Guinea pig	[59]
	CCHFV	Type I IFN receptor knockout mice	[60]
	RVFV	Mice, sheep, lambs, suckling mice	[45,56–58,61]
Viral genome rearrangement	LCMV	Mice	[64,66–67]
	MACV	Guinea pig	[65]
	JUNV	Mice	[64]
	RVFV	Mice, lambs, pregnant ewes	[62–63]
Chimeric viral genes	LASV	NHP	[71]
	MACV	Mice	[69–70]
	LACV	Mice, NHP	[47]
	RVFV	Mice	[68]