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. Author manuscript; available in PMC: 2021 Dec 21.
Published in final edited form as: Curr Biol. 2020 Oct 15;30(24):4896–4909.e6. doi: 10.1016/j.cub.2020.09.045

Figure 2. Whole-VNC functional imaging analysis reveals a decision-associated CNS region.

Figure 2.

(A) The steps for identifying nociceptor-activated CNS regions. Arrows point to different nociceptor-activated CNS regions on representative z sections. N = 50 larvae. In all neural activity atlases throughout this study, red, green, yellow, and blue represent PMC, ADs, PDs, and VMs, respectively, unless otherwise noted. The anterior side of the VNC is to the left. The top-left and bottom-right panels are the dorsal-most and ventral-most z sections, respectively.

(B) The seven nociceptor-activated CNS regions.

(C) The neural activity levels in different nociceptor-activated CNS regions at each second after nociceptor stimulation (details are in Figure S2). Each dot represents the mean activity level in a CNS region. Data is from (A).

(D) Neural activity probability (indicated by Z-score) of distinct nociceptor-activated regions in each SON activation group. Each dot represents a larva. N = 20 larvae per SON activation group. Two-way ANOVA (Dunnett’s post hoc). Throughout this study, n.s.: P >= 0.05, no statistical difference; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

(E) Pearson’s correlation coefficient between neural activity level of distinct nociceptor-activated CNS regions and escape probability. N = 675 larvae for neural activity and 2,294 for paired behaviors (from 20 experimental groups in this study). Details are in Figure S3.

See also Figures S1, S2 and S3, Video S1 and Table S1.