Skip to main content
. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: Nat Microbiol. 2020 Nov 2;6(1):19–26. doi: 10.1038/s41564-020-00798-4

Fig. 3. The L,D transpeptidase ldt2 is required for covalent attachment of BbpA and BbpB to PG.

Fig. 3.

a. Immunoblots of stationary phase whole cell lysate from wild-type (WT), Δldt, and complement strains, lysozyme-digested or undigested, and probed with BbpA (top) or LimB (bottom) specific antibodies. Data are representative of n=3 independent experiments. Molecular weight markers are shown to left of the blot. b. Extracted Ion Chromatograms (XIC) of precursor masses (m/z) corresponding to PG-BbpA (AEmGGPDYVPAPS, m/z = 906.909, z = 2), PG-ala-BbpA (AEmAGGPDYVPAPS, m/z = 942.427, z = 2), PG-BbpB (AEmGGPDIPM, m/z = 769.843, z = 2) identified in PG from WT, Δldt2, and Δldt2 complement strains. c. WT and Δldt deletion mutants were grown in ACCM-D for 14 days and imaged by cryo-EM. OM vesiculation was quantified by imaging ≥50 bacterial cells per strain and expressed as the percentage of bacteria with vesicles attached to the OM. d. Ultrastructure of WT and the Δldt2 deletion mutant imaged by cryo-EM. Arrows denote OM vesiculation.