Extended Data Fig. 9. The L,D transpeptidase ldt2 is required for covalent attachment of BbpA and BbpB to PG.
XIC analysis was performed on PG that was extracted from WT and Δldt mutant strains and analyzed by MS/MS. XIC’s of precursor masses (m/z) corresponding to PG-bound BbpA (AEmGGPDYVPAPS, m/z = 906.909, z = 2), ala-BbpA (AEmAGGPDYVPAPS, m/z = 942.427, z = 2), and BbpB (AEmGGPDIPM, m/z = 769.843, z = 2) are shown.