FIGURE 5.

SOX4 was identified as a direct target of miR‐212. A, Computer prediction of the 3′‐UTR of SOX4 mRNA contained a target site for miR‐212. B, Transfection efficiency of miR‐212 mimics. C, Luciferase activity assay revealed that miR‐212 suppressed SOX4 3′‐UTR‐wt luciferase activity, while it had no effect on SOX4 3′‐UTR luciferase activity compared to control in SNU‐16 cells. D, The mRNA expression of SOX4 was examined by qRT‐PCR analysis in SNU‐16 cells. E and F, The protein level of SOX4 was detected by western blot after transfected with miR‐212 mimics or control in SNU‐16 cells. GAPDH was chosen as a loading control. *P < .05