KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-β-tubulin	Sigma-Aldrich	Cat# T7816; RRID: AB_261770
Mouse monoclonal anti-DsRed2	Santa Cruz Biotechnology	Cat# sc-101526; RRID: AB_1562589
Rabbit polyclonal anti-Spc25	Atlas Antibodies	Cat# HPA047144; RRID: AB_2679952
Rabbit polyclonal anti-Nuf2	Bethyl	Cat# A304–319A; RRID: AB_2620515
Mouse monoclonal anti-GFP	Takara Bio	Cat# 632381; RRID: AB_2313808
Goat monoclonal anti-mouse, horseradish peroxidase conjugated	Sigma-Aldrich	Cat# A4416; RRID: AB_258167
Goat monoclonal anti-rabbit, horseradish peroxidase conjugated	Sigma-Aldrich	Cat# A4914; RRID: AB_258207
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Nocodazole	Fisher	Cat# AC358240100; CAS: 31430-18-9
Taxol	Fisher	Cat# NC9507351; CAS: 33069-62-4
ZM447439	Fisher	Cat# 508279
Doxycycline	Fisher	Cat# BP26531; CAS: 10592-13-9
Thymidine	Millipore	Cat# 6060; CAS: 50-89-5
Puromycin	Fisher	Cat# ICN19453910
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
HeLa A12	[18]	N/A
Experimental Models: Organisms/Strains
S. cerevisiae: Strain background: YEF473	ATCC	ATTC:
Oligonucleotides
For a list of all siRNAs used in this study, see Supplementary Table 3
Recombinant DNA
pEM784 – pCAGGS-nls-Cre	[18]	N/A
pERB131 – Mis12-GFP-FKBPx3; inducible: mCh-Mps1	Lampson lab	N/A
For plasmids for the expression of FRET pairs in HeLa A12 cells, see Supplementary Table 2	This study	N/A
For plasmids for the expression of FRET pairs in S. cerevisiae, please see the accompanying reference	[3]	N/A
Software and Algorithms
Prism	Graphpad	Ver. 8
MATLAB	Mathworks	Ver. 2017b
VistaVision	ISS	Ver. 4.0
Other
DMEM	Thermo Fisher	N/A
Lipofectamine 3000	Life Technologies	Cat# L3000008
Lipofectamine RNAiMAX	Life Technologies	Cat# 13778075