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. Author manuscript; available in PMC: 2021 May 9.
Published in final edited form as: Nat Microbiol. 2020 Nov 9;6(1):34–43. doi: 10.1038/s41564-020-00808-5

Extended Data Fig. 2. The complex cleaves native PG oligomers to defined lengths similarly to synthetic PG oligomers.

Extended Data Fig. 2

To prepare linear glycan strands, extracted S. aureus Lipid II was incubated with transpeptidase-inactive PBP2S398G and then the SagB-SpdC complex or individual hydrolases (SagB or SagA). Reactions were quenched and products were then labeled with biotin-D-lysine (BDL) in the presence of the E. faecalis PBPX and visualized using a western blot method with HRP-streptavidin detection19,23,29.