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. Author manuscript; available in PMC: 2021 May 9.
Published in final edited form as: Nat Microbiol. 2020 Nov 9;6(1):34–43. doi: 10.1038/s41564-020-00808-5

Extended Data Fig. 3. The catalytic glutamate of SagB (E155), but not conserved CAAX protease residues in SpdC (E135, R139, H210)5, is necessary to complement shared cell wall phenotypes of sagB and spdC mutants.

Extended Data Fig. 3

Spot dilution series of S. aureus strains with mutations in sagB or spdC were plated on TSA or TSA containing tunicamycin or lysostaphin. Plates shown on the right are replicates of those on the left but the agar contains the inducer, anhydrotetracycline.