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. 2020 Dec 22;11:6439. doi: 10.1038/s41467-020-20239-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Anti-TBPL2 immunoprecipitation followed by mass spectrometry (IP-MS) analysis (three technical replicates) of NIH3T3 overexpressing TBPL2 (NIH3T3-II10) whole-cell extracts (WCE). The colour code for the different proteins or complexes is indicated on the right. b Anti-TBP IP-MS analysis (three technical replicates) of NIH3T3-II10 WCE. Colour legend for the different proteins is the same as in (a). c Western blot of a Superose 6 gel filtration analysis of NIH3T3-II10 WCE probed with anti-TAF6 (top panel), anti-TBPL2 and anti-TFIIA-α (middle panels) and anti-TBP (bottom panel) antibodies. Fraction numbers are shown above each lane, and the elution of known molecular mass markers is indicated above the panels. The pooled fractions used for mass spectrometry analysis are indicated in red. d Anti-TBPL2 IP-MS analysis (three technical replicates) of the gel filtration fraction indicated in (c). The colour code for the different proteins or complexes is indicated on the right. NSAF normalised spectral abundance factor. e Schematic representation of the fundamental differences existing between TBPL2- and TBP-containing complexes in growing oocytes and NIH3T3-II10 cells. In (a, b, and d) grey dots indicate replicates and error bars, +/− standard error of the mean.