FIGURE 3.

MgH₂-CBS triggers H₂S accumulation. (A) Cut flower stems were incubated in untreated (control) and treatment solutions containing 0.1 g L^–1 MgH₂-CBS, 600-μM NaHS, 10-mM HT (a scavenger of H₂S), alone or their combinations for 3 days. Afterward, epidermis of petals was loaded with 20-μM WSP5 (an H₂S fluorescent probe) and detected by laser scanning confocal microscopy (scale bar = 200 μm). Bright-field images corresponding to the fluorescent images were at the bottom right corner. (B) Relative fluorescence was also presented as values relative to control. Mean and SE values were calculated. At least five sections per sample were determined, and three samples in each treatment were used. Bars with different letters denoted significant differences in comparison with control at P < 0.05, according to Duncan’s multiple range test.