Figure 1.

Single-cell atomic force microscopy (sc-AFM) analysis of the adhesive interactions between Streptococcus and Candida albicans. (a) Schematic diagram of sc-AFM. AFM tipless cantilever probes were functionalized with Streptococcus mutans (or Streptococcus gordonii), and C. albicans cells were immobilized on a coverslip. Fluorescent image of S. mutans (b1) or S. gordonii (b2) functionalized AFM probes. (c1) Confocal image of immobilized C. albicans on the poly-L-lysine-coated coverslip. (c2) Total Internal Reflection Fluorescence-AFM image of glucans formed on immobilized C. albicans.