Fig. 3.

DeepSTORM3D solves the 3D dense emitter localization using a neural network. a Top: Example experimentally measured frame. Each shape (PSF) is a single fluorescent molecule. Bottom: Rendered frame from recovered emitters by the neural net. Scale bar: 5 μm. b Super-resolved mitochondria image from thousands of frames. Color encodes depth. Scale bar: 5 μm. c An optimal phase mask (left) and PSF (right) for dense emitters in 3D, learned by the neural net. Scale bar: 3 μm. Figure adapted from Nehme et al. (2020b)